pubmed
Cell line-matched reference enables high-precision functional genomics.
Corda L; Volpe E; Dallali H; Di Tommaso E; Colantoni A; Guarracino A; Chittoor SS; Capulli M; Tassone E; Giunta S
Scientists found that using a special type of genome, called an "isogenomic" diploid reference genome, can make studying and editing human cell DNA much better. They used it for a well-known cell line and discovered differences in DNA and other cell parts that were specific to certain haplotypes and chromosomes. This approach helps improve the accuracy of genetic studies and suggests that more cell lines should have their own custom genomes.
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